Genencor International, Inc., a part of Danisco US Inc., has completed a commercial license agreement for the use of the Red/ET recombination technology from Gene Bridges GmbH (Heidelberg, Germany; www.genebridges.com). Under the terms of the agreement, Gene Bridges has licensed the Red/ET technology to Genencor for the purpose of genetically engineering recombinant microorganisms for use in industrial applications. No financial details were disclosed.
Red/ET Recombineering is one simple and powerful tool to modify plasmids, BACs, and even E. coli chromosomes, and is said to be a revolutionary method for DNA engineering. Recombineering with Red/ET allows unlimited cloning, subcloning, and modification of DNA at any chosen position. It permits precise engineering of DNA molecules of any size, including very large ones such as BACs or the E. coli chromosome. Recombineering DNA using Red/ET has a number of advantages over conventional methods: independent of restriction sites; no size limits; no unwanted mutations; and speed.